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27 The cryopreserved cells were cultured in Iscoves modified Dulbecos medium supplemented with 20% FBS, insulin, selenium, transferrin, 10 ng/ml of interleukin-6, insulin-like growth factor-1 and vascular endothelial growth factor at 5% O 2 for 1 week before sorting primary MM cells

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Toxicology and applied pharmacology 257 , 338348, doi: 10.1016/j.taap.2011.09.017 (2011)

Therefore the MTHFR enzyme supports not only fertility, but weight, immunity and production of serotonin, dopamine and melatonin the hormones that regulate moods and sleep

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The migration pattern of HUVEC on the culture plate was observed, and the widths of the scratch before and after migration were compared among the groups using ImageJ